Sheep antibody to SUMO-1 (6-21): whole serumS-064-100
Catalog No#: S-064-100
Product Name: Sheep antibody to SUMO-1 (6-21): whole serum
Alternative Name: Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
Product Type: Polyclonal Antibody
Description: SUMO-1 binds to a wide range of target proteins as part of a post-translational modification system. Unlike ubiquitin, it does not seem to target protein for degradation, but is involved in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis, apoptosis, protein stability and signal transduction. SUBUNIT: Covalently attached to a number of proteins such as PmL, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain. PTM: Cleavage of the last four amino acids of the carboxy-terminus of the precursor form by SENP1 or SENP2 is necessary for function. Several pseudogenes have been reported as well as a number of alternatively spliced isoforms.
Size: 100 uL
Applications: WB; IHC, Frozen;
Recommended Dilutions: IHC, WB. A dilution of 1:2000 to 1:4000 is recommended for immunohistochemistry and 1:4000 to 1:8000 for western blot. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunogen: A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Purity: Whole serum
Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Species Reactivity: This antiserum is known to cross react with Rat and Human SUMO-1.
Specification: This antiserum recognises human SUMO-1 and not ubiquitin. | This antiserum is known to cross react with rat and human SUMO-1.
Shipping Conditions: 25°C (Ambient)
Storage Conditions: After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiration Date: 12 months after purchase
Datasheet Link: http://www.biosensis.com/pdf_datasheet.php?products_id=192
Product Link: http://www.biosensis.com/product_info.php/sheep-antibody-sumo-whole-serum-p-192