Sheep polyclonal antibody to dephosphorylated Catenin beta (28-46): Affinity purified
Catalog No#: S-1233-100
Product Name: Sheep polyclonal antibody to dephosphorylated Catenin beta (28-46): Affinity purified
Alternative Name: Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; _-Catenin;
Product Type: Polyclonal Antibody
Description: Catenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.
Size: 100 ug
Applications: WB; IHC, Frozen; IC;
Recommended Dilutions: Western Blotting (WB) and Immunocytochemistry. A concentration of 2.0 µg/mL is recommended for WB. Human Catenin beta (isoform 1) has a predicted length of 781 residues and MW of 86 kDa. It has been observed as a 92 kDa band in SDS-reducing gels. A concentration of 4.0 µg/mL is recommended for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Formation: Lyophilized from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.
Immunogen: A synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.
Purity: Affinity purified
Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Species Reactivity: Human; Mouse; Rat;
Specification: The specificity of this antibody has been confirmed by WB against the antigen. | Human; mouse; rat;
Shipping Conditions: 25°C (Ambient)
Storage Conditions: At least 12 months after purchase at 2 - 4 °C (lyophilized formulations).After reconstitution, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Expiration Date: 12 months after purchase
Datasheet Link: http://www.biosensis.com/pdf_datasheet.php?products_id=724
Product Link: http://www.biosensis.com/product_info.php/sheep-polyclonal-antibody-dephosphorylated-catenin-beta-affinity-purified-p-724