DNA Ligase4 Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Research Areas: Cancer, Epigenetics and Nuclear Signaling
Applications: WB,IHC-p,IF,ELISA
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000
Size: 200 uL
Concentration: 1mg/mL
Swissprot: P49917
Gene Accession:
Reactivity: Human
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from the Internal region of human DNA Ligase IV
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 104kDa
Tissue Specificity/Positive Control: Testis, thymus, prostate and heart.
Cellular Localization: Nucleus.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-dna_ligase4_polyclonal_antibody-27537.html
Manual Link: https://www.elabscience.com/viewpdf-27537-elabscience-E-AB-31212.PDF