Human A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: A Disintegrin And Metalloproteinase With Thrombospondin 5 Alternative Names: ADMP-2; Aggrecanase-2 Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Enzyme & Kinase; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: tissue homogenates, cell lysates and other biological fluids Organism Species: Homo sapiens (Human) Detection Sensitivity: < 0.112ng/mL Detection Range: 0.312-20ng/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEK205Hu.html