Human TNF-a (Tumor Necrosis Factor Alpha) CLIA Kit

Supplier: Elabscience
Product Type: CLIA
Size: 96T
Description: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-? . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-? and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-?, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNF-?. The concentration of Human TNF-? in the samples can be calculated by comparing the RLU of the samples to the standard curve.
Target Name: TNF-?
Target Synonym: DIF; TNF-alpha; TNFA; TNFSF2
UNIProt ID: P01375
Target Species: Human
Type: Sandwich
Detection Range: 7.813--500 pg/mL
Detection Sensitivity: 4.688 pg/mL
Tested Samples: Serum, Plasma, Cell supernatant
Product Link: http://www.elabscience.com/p-human_tnf_?_(tumor_necrosis_factor_alpha)_clia_kit-15091.html
Manual Link: http://www.elabscience.com/viewpdf-15091-Elabscience-E-CL-H0108.pdf