Supplier: Cloud-Clone Corp.
Product Type: ELISA Kit
Size: 96T
Item Name: Thyroid Stimulating Hormone
Alternative Names: Thyrotropin
Description: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thyroid Stimulating Hormone (TSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thyroid Stimulating Hormone (TSH) and unlabeled Thyroid Stimulating Hormone (TSH) (Standards or samples) with the pre-coated antibody specific to Thyroid Stimulating Hormone (TSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample.
Research Area: Endocrinology;Hormone metabolism;
Assay Type: Competitive Inhibition
Applications: Enzyme-linked immunosorbent assay for Antigen Detection.
Sample Types: serum, plasma and other biological fluids
Organism Species: Mus musculus (Mouse)
Detection Sensitivity: < 19.2pg/mL
Detection Range: 49.4-4000pg/mL
Assay Length: 2 hours
Product Link: http://www.cloud-clone.com/products/CEA463Mu.html