Mouse Toll Like Receptor 4 (TLR4) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Toll Like Receptor 4 Alternative Names: CD284; TOLL; HToll Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 4 (TLR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 4 (TLR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 4 (TLR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor 4 (TLR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity;Pulmonology; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids Organism Species: Mus musculus (Mouse) Detection Sensitivity: < 0.054ng/mL Detection Range: 0.156-10ng/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEA753Mu.html