Phospho-SMC1A (Ser966) Polyclonal Antibody

Supplier: Elabscience
Product Category: Phospho/Acetyl/Methyl
Description: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Research Areas: Cancer, Cell Biology
Size: 200 uL
Concentration: 1mg/mL
Applications: WB,IHC-p,ELISA
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000
Clonality: Polyclonal
Clone No:
SwissProt: Q14683
Gene ID: 8243
Reactivity: Human,Mouse,Rat
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from human SMC1 around the phosphorylation site of S966.
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Conjugation: Unconjugated
Observed MW: 160kDa
Storage: Store at -20C. Avoid freeze / thaw cycles.
Cellular Localization: Nucleus. Chromosome. Chromosome>centromere>kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.

Product Link: https://www.elabscience.com/p-phospho_smc1a_(ser966)_polyclonal_antibody-41837.html
Manaul Link: https://www.elabscience.com/viewpdf-41837-elabscience-E-AB-21969.PDF