Mouse Monoclonal Antibody to APAF1

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; ICC: 1/100 - 1/500; FCM: 1/200 - 1/400; IHC: N/A
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 2H9A1
Isotype: Mouse IgG2b
Immunogen: Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.
Formulation: Purified antibody in PBS with 0.05% sodium azide
MW: 141.8kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=4529
Reference: 1.Tumour Biol. 2014 Mar;35(3):2211-8.2.Cancer Sci. 2011 Jan;102(1):267-74.

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