Rat Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Vascular Cell Adhesion Molecule 1 Alternative Names: CD106; INCAM-100; L1CAM Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Cell Adhesion Molecule 1 (VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Organism Species: Rattus norvegicus (Rat) Detection Sensitivity: < 0.45ng/mL Detection Range: 1.25-80ng/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEA547Ra.html