Recombinant Mouse Tumor Necrosis Factor Receptor I Protein

Supplier: Elabscience Product Name: Recombinant Mouse Tumor Necrosis Factor Receptor I Protein Catalog No. PKSM041215 Product Type: recombinant protein Size: 50 ug Activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized human TNFa at 10 ?g/mL (100 ?l/well) can bind  biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.28 ?g/mL.3. Immobilized mouse TNFa at 10 ?g/mL (100 ?l/well) can bind  biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.13 ?g/mL.4. Measured by its ability to inhibit the TNF?-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-1.5 ?g/mL in the presence of 0.1 ng/mL recombinant mouse TNF?. Protein Construction: Recombinant Mouse Tumor Necrosis Factor Receptor I is produced by our E.coli expression system and the target gene encoding Ile22-Ala212 is expressed. Sequence: Met 1-Ala 212 Fusion Tag: Accession: NP_ 035739.2 Species: Mouse Expressed Host: E.coli Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 21.2 kDa Applied MM: 20-25kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of 20mM PB?150mM NaCl,pH7.4. Reconstitution: Please refer to it for detailed information. Background: Tumor necrosis factor receptor superfamily member 1A (Tnfrsf1a) is a member of the tumor necrosis factor receptor superfamily. Tnfrsf1a is one of the major receptors for the tumor necrosis factor-alpha. It can activate the transcription factor NF-?B, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the human genetic disorder called tumor necrosis factor associated periodic syndrome (TRAPS) or periodic fever syndrome