Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000
Size: 100 ul, 1mg/ml
Species Reactivity: Human, Mouse
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of CHK1 expressed in E. Coli.
Formulation: Purified antibody in PBS containing 0.03% sodium azide.
Storage: 4C; -20C for long term storage
Spplier link: http://www.promab.com/index.php?main_page=product_info&products_id=61
Reference: 1. EMBO J. 1998. 16: 545-554. ; 2. Science 1998. 282: 1893-1897.