Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin), thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of cTnI expressed in E. Coli.
Formulation: Ascitic fluid containing 0.03% sodium azide.
Storage: 4C; -20C for long term storage
Spplier link: http://www.promab.com/index.php?main_page=product_info&products_id=77
Reference: 1. Cummins B and Cummins P, J Mol Cell Cardiol, 1987, 19(10):999-1010. ; 2. Cummins B, Auckland ML, and Cummins P, Am Heart J, 1987, 113(6):1333-44. ; 3. Darnell J, Lodish H, and Baltimore D, Molecular Cell Biology, New York, NY: Scientific American Books, 1986, 827-8. ; 4. Larue C, Defacque-Lacquemant H, Calzolari C, et al, Mol Immunol, 1992, 29(2):271-8. ; 5. Adams JE III, Bodor GS, Davila-Roman VG, et al, Circulation, 1993, 88(1):101-6. ;