Figure 1: Western blot analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).
Mouse Monoclonal Antibody to GFP

Mouse Monoclonal Antibody to GFP

20144

Regular price $264.00 $224.00 You Pay

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: N/A
Clone: 4B10B2;
Isotype: Mouse IgG2a
Immunogen: Purified recombinant fragment of GFP expressed in E. Coli.
Formulation: Purified antibody in PBS containing 0.03% sodium azide.
MW: 27kDa
Storage: 4C; -20C for long term storage
Spplier link: http://www.promab.com/index.php?main_page=product_info&products_id=157
Reference: 1. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404. ; 2. Gene Expr Patterns. 2007, Jan,7(1-2):124-30. ; 3. Eur J Cell Biol. 2006, Sep,85(9-10):1059-68. ;