Mouse Monoclonal Antibody to NANOG

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 1E6C4;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli. ;
Formulation: Ascitic fluid containing 0.03% sodium azide. ;
MW: 35kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=278
Reference: 1. Genes Cells. 2006 Sep;11(9):1115-23. ; 2. Mol Biol Cell. 2007 May;18(5):1543-53.

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