Figure 1: Western blot analysis using CDH1 mouse mAb against LNCAP (1)?A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.
Mouse Monoclonal Antibody to CDH1

Mouse Monoclonal Antibody to CDH1

30121

Regular price $308.00 $262.00 You Pay

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.Tissue specificity: Non-neural epithelial tissues.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human; Mouse; Monkey
Clone: 7H12;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human CDH1 expressed in E. Coli.
Formulation: Ascitic fluid containing 0.03% sodium azide. ;
MW: 135kDa
Storage: 4C; -20C for long term storage
Spplier link: http://www.promab.com/index.php?main_page=product_info&products_id=520
Reference: 1. Nat Genet. 2009 Dec;41(12):1330-4. ; 2. Zhonghua Zhong Liu Za Zhi. 2009 Jul;31(7):515-9. ; 3. J Biol Chem. 2010 Feb 26;285(9):6658-69.