Product Type: Antibody
Descritpion: Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
Research Areas: Cell Biology
Recommended Dilutions: IHC 1:25-100, ELISA 1:2000-5000
Size: 200 uL
Gene Accession: NP_060255
Immunogen: Synthetic peptide of human SPDL1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Tissue Specificity/Positive Control:
Cellular Localization: Cytoplasm>cytoskeleton>centrosome. Chromosome>centromere>kinetochore. Nucleus. Cytoplasm>cytoskeleton>spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
Storage: Store at -20C. Avoid freeze / thaw cycles.