Di-Methyl-P53 (Lys370) Polyclonal Antibody

Supplier: Elabscience
Product Category: Phospho/Acetyl/Methyl
Description: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling
Size: 200 uL
Concentration: 1mg/mL
Applications: WB,IHC-p,IF,ELISA
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-300, IF 1:50-1:200, ELISA 1:10000-1:20000
Clonality: Polyclonal
Clone No:
SwissProt: P04637
Gene ID: 7157
Reactivity: Human
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from human p53 around the di-methylation site of K370.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Conjugation: Unconjugated
Observed MW: 53kDa
Storage: Store at -20C. Avoid freeze / thaw cycles.
Cellular Localization: Cytoplasm; Cytoplasm. Nucleus. Nucleus>PML body. Endoplasmic reticulum. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2; Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli; Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells; Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm; Nucleus. Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.

Product Link: https://www.elabscience.com/p-di_methyl_p53_(lys370)_polyclonal_antibody-26416.html
Manaul Link: https://www.elabscience.com/viewpdf-26416-elabscience-E-AB-30091.PDF