Product Type: Antibody
Descritpion: Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Developmental biology, Stem cells
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300 ELISA 1:20000
Size: 200 uL
Immunogen: Synthesized peptide derived from the C-terminal region of human TERT.
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Calculated MW: 127kDa
Tissue Specificity/Positive Control: Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T lymphocytes, and at a low to undetectable level in peripheral blood T lymphocytes.
Cellular Localization: Nucleus>nucleolus. Nucleus>nucleoplasm. Nucleus. Chromosome>telomere. Cytoplasm. Nucleus>PML body. Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT.
Storage: Store at -20C. Avoid freeze / thaw cycles.