PAK1 Polyclonal AntibodyE-AB-32498
Product Type: Antibody
Descritpion: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Research Areas: Cancer, Cell Biology, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000
Size: 200 uL
Immunogen: Synthesized peptide derived from human PAK? around the non-phosphorylation site of Thr212.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Calculated MW: 61kDa
Tissue Specificity/Positive Control: Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321).
Cellular Localization: Cytoplasm. Cell junction>focal adhesion. Recruited to focal adhesions upon activation.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-pak1_polyclonal_antibody-28823.html
Manual Link: https://www.elabscience.com/viewpdf-28823-elabscience-E-AB-32498.PDF