Product Type: Antibody
Descritpion: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (?, ?I, ?II, ?, ?, ?, ?, ?, ?, ?, ?, and ?). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance,the kinase activities of PKC ? and ? are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.
Research Areas: Cancer, Cardiovascular, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000
Size: 200 uL
Immunogen: Synthesized peptide derived from human PKC around the non-phosphorylation site of Thr497.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Calculated MW: 76-83kDa
Tissue Specificity/Positive Control:
Cellular Localization: Cytoskeleton,Cytosol,Endoplasmic reticulum,endoplasmic reticulum,Golgi apparatus,Golgi apparatus,Mitochondrion,Nucleus,Plasma Membrane,Other locations:cell periphery,cytoplasm,perinuclear region of cytoplasm
Storage: Store at -20C. Avoid freeze / thaw cycles.