Phospho-SMAD3 (Ser425) Monoclonal AntibodyE-AB-51043
Product Category: Phospho/Acetyl/Methyl
Description: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction, Stem cells
Size: 200 uL
Recommended Dilutions: IHC 1:50-300
Clone No: Clone:4C5
Gene ID: 4088
Immunogen: Synthetic Peptide of Phospho-Smad3 (Ser425)
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Purification: Protein A purification
Storage: Store at -20C. Avoid freeze / thaw cycles.
Cellular Localization: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236).
Product Link: https://www.elabscience.com/p-phospho_smad3_(ser425)_monoclonal_antibody-41300.html
Manaul Link: https://www.elabscience.com/viewpdf-41300-elabscience-E-AB-51043.PDF