Human C4a (Complement Component 4a) CLIA Kit

Supplier: Elabscience
Product Type: CLIA
Size: 96T
Description: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C4a . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C4a. The concentration of Human C4a in the samples can be calculated by comparing the RLU of the samples to the standard curve.
Target Name: C4a
Target Synonym: C4AD; C4S; CO4; CPAMD2; RG
UNIProt ID: P0C0L4
Target Species: Human
Type: Sandwich
Detection Range: 0.313--20 ng/mL
Detection Sensitivity: 0.188 ng/mL
Tested Samples: Serum, Plasma, Cell supernatant
Product Link: http://www.elabscience.com/p-human_c4a_(complement_component_4a)_clia_kit-15150.html
Manual Link: http://www.elabscience.com/viewpdf-15150-Elabscience-E-CL-H0173.pdf