Product Type: CLIA
Description: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-? . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-? and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-?, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TNF-?. The concentration of Mouse TNF-? in the samples can be calculated by comparing the RLU of the samples to the standard curve.
Target Name: TNF-?
UNIProt ID: P09225
Target Species: Mouse
Detection Range: 7.813--500 pg/mL
Detection Sensitivity: 4.688 pg/mL
Tested Samples: Serum, Plasma, Cell supernatant
Product Link: http://www.elabscience.com/p-mouse_tnf_?_(tumor_necrosis_factor_?)_clia_kit-16900.html
Manual Link: http://www.elabscience.com/viewpdf-16900-Elabscience-E-CL-M0671.pdf