Product Type: CLIA
Description: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-? . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-? and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-?, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TNF-?. The concentration of Rat TNF-? in the samples can be calculated by comparing the RLU of the samples to the standard curve.
Target Name: TNF-?
Target Synonym: DIF; TNF-alpha; TNFA; TNFSF2
UNIProt ID: P16599
Target Species: Rat
Detection Range: 15.625--1000 pg/mL
Detection Sensitivity: 9.375 pg/mL
Tested Samples: Serum, Plasma, Cell supernatant
Product Link: http://www.elabscience.com/p-rat_tnf_?_(tumor_necrosis_factor_alpha)_clia_kit-16955.html
Manual Link: http://www.elabscience.com/viewpdf-16955-Elabscience-E-CL-R0019.pdf