Rat CYP7A1 (Cholesterol 7-Alpha-Hydroxylase) CLIA Kit

Supplier: Elabscience
Product Type: CLIA
Size: 96T
Description: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CYP7A1 . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CYP7A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CYP7A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CYP7A1. The concentration of Rat CYP7A1 in the samples can be calculated by comparing the RLU of the samples to the standard curve.
Target Name: CYP7A1
Target Synonym:
UNIProt ID: P18125
Target Species: Rat
Type: Sandwich
Detection Range: 3.125--200 ng/mL
Detection Sensitivity: 1.875 ng/mL
Tested Samples: Serum, Plasma, Cell supernatant
Product Link: http://www.elabscience.com/p-rat_cyp7a1_(cholesterol_7_alpha_hydroxylase)_clia_kit-17575.html
Manual Link: http://www.elabscience.com/viewpdf-17575-Elabscience-E-CL-R0725.pdf