Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat PACAP-27. During the reaction, Rat PACAP-27 in the sample or standard competes with a fixed amount of Rat PACAP-27 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PACAP-27. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Rat PACAP-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: PACAP-27
Target Synonmy: Pituitary Adenylate Cyclase Activating Polypeptide 27
UNIProt ID:
Target Species: Rat
Detection Range: 7.813--500pg/mL
Sensitivity: 4.688pg/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-rat_pacap_27(pituitary_adenylate_cyclase_activating_polypeptide_27)_elisa_kit-17829.html

Manaul Link: http://www.elabscience.com/viewpdf-17829-Elabscience-E-EL-R1436.pdf