Recombinant Mouse Intercellular adhesion molecule 2 Protein

Supplier: Elabscience Product Name: Recombinant Mouse Intercellular adhesion molecule 2 Protein Catalog No. PKSM041208 Product Type: recombinant protein Size: 50 ug Activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When 5 x 10E4 cells/well are added to rmICAM1 coated plates (12.5 ?g/mL with 100 ?L/well), > 30% cells will adhere after 1 hour incubation at 37?. Protein Construction: Recombinant Mouse Intercellular adhesion molecule 2 is produced by our Mammalian expression system and the target gene encoding Lys23-Gln222 is expressed with a Fc tag at the C-terminus. Sequence: Met 1-Gln 222 Fusion Tag: C-Fc Accession: NP_034624.1 Species: Mouse Expressed Host: Human Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 49.7 kDa Applied MM: 70-90 kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of 20mM Tris,150mM NaCl,pH8.0. Reconstitution: Please refer to it for detailed information. Background: ICAM-2 is a 55-65 kD transmembrane glycoprotein possessing 2 extracellular Ig domains, a single transmembrane domain, and a short 26-amino acid cytoplasmic domain. ICAM-2 is expressed on most leukocytes, and is strongly expressed on vascular endothelial cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.