Recombinant Mouse Programmed Cell Death 1 Ligand 1 Protein

Supplier: Elabscience Product Name: Recombinant Mouse Programmed Cell Death 1 Ligand 1 Protein Catalog No. PKSM041248 Product Type: recombinant protein Size: 50 ug Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD1-L1 at 1 ?g/ml (100 ?l/well) can bind mouse PD1 with a linear range of 6.25-400 ng/ml. Protein Construction: Recombinant Mouse Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a 6His tag at the C-terminus. Sequence: Met 1-Thr 238 Fusion Tag: C-6His Accession: NP_068693.1 Species: Mouse Expressed Host: Human Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 25.6 kDa Applied MM: 39-58 kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Reconstitution: Please refer to it for detailed information. Background: Mouse Programmed cell death 1 ligand 1(Cd274?PD-L1), is a member of the growing B7 family of immune proteins.It involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.B7-H1 has been identified as one of two ligands for programmed death1 (PD1), a member of the CD28 family of immunoreceptors. B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs.B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN gamma stimulation. Interaction of B7-H1 with PD1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.