Recombinant Mouse Bone Morphogenetic Protein Receptor Type IA/Activin Receptor-like Kinase 3 Protein

Supplier: Elabscience Product Name: Recombinant Mouse Bone Morphogenetic Protein Receptor Type IA/Activin Receptor-like Kinase 3 Protein Catalog No. PKSM041249 Product Type: recombinant protein Size: 50 ug Activity: Measured by its ability to inhibit recombinant human BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.5-2 ?g/ml in the presence of 50 ng/ml of recombinant human BMP4. Protein Construction: Recombinant Mouse Bone Morphogenetic Protein Receptor Type IA/Activin Receptor-like Kinase 3 is produced by our Mammalian expression system and the target gene encoding Gln24-Arg152 is expressed with a Fc, 6His tag at the C-terminus. Sequence: Gln24-Arg152 Fusion Tag: C-Fc-6His Accession: P36895 Species: Mouse Expressed Host: Human Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 42.2 kDa Applied MM: 55-60&120kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of 20mM PB,150mM NaCl,pH7.4. Reconstitution: Please refer to it for detailed information. Background: ALK-3 is a type I receptor for bone morphogenetic proteins (BMPs) which belong to the protein kinase superfamily, TKL Ser/Thr protein kinase family and TGFB receptor subfamily. The BMP receptors consists of the type I receptors BMPR1A and BMPR1B and the type I I receptor BMPR2. Seven known type I serine/threonine kinases and five mammalian type II serine/threonine kinase receptors function in TGF-beta superfamily signal transduction. The downstream molecules of the type I BMP receptors include the Smad (Smad1, 5 and 8) proteins that are phosphorylated in a ligand-dependent manner, and relay the BMP signal from the receptors to target genes in the nucleus. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. ALK-3 contains a GS domain and a protein kinase domain. ALK-3 is widely expressed. Defects in BMPR1A gene are a cause of a significant proportion of cases of Juvenile polyposis syndrome (JPS).