Cattle Leptin (LEP) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Leptin Alternative Names: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin (LEP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leptin (LEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leptin (LEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leptin (LEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Organism Species: Bos taurus; Bovine (Cattle) Detection Sensitivity: < 0.061ng/mL Detection Range: 0.156-10ng/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEA084Bo.html