Mouse Procollagen I N-Terminal Propeptide (PINP) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Procollagen I N-Terminal Propeptide Alternative Names: P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen I N-Terminal Propeptide (PINP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen I N-Terminal Propeptide (PINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen I N-Terminal Propeptide (PINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Metabolic pathway;Hepatology;Bone metabolism; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: serum, plasma and other biological fluids Organism Species: Mus musculus (Mouse) Detection Sensitivity: < 19.37pg/mL Detection Range: 46.88-3000pg/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEA957Mu.html