Supplier: Cloud-Clone Corp.
Product Type: ELISA Kit
Size: 96T
Item Name: Corticosteroid Binding Globulin
Alternative Names: SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase; Antitrypsin)
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticosteroid Binding Globulin (CBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corticosteroid Binding Globulin (CBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticosteroid Binding Globulin (CBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticosteroid Binding Globulin (CBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Endocrinology;
Assay Type: Double-antibody Sandwich
Applications: Enzyme-linked immunosorbent assay for Antigen Detection.
Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Organism Species: Rattus norvegicus (Rat)
Detection Sensitivity: < 6.9ng/mL
Detection Range: 15.6-1000ng/mL
Assay Length: 2 hours
Product Link: http://www.cloud-clone.com/products/SEB226Ra.html