Mouse Bcl2 Associated X Protein (Bax) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Bcl2 Associated X Protein Alternative Names: BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4 Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein (Bax). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Associated X Protein (Bax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein (Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated X Protein (Bax) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Signal transduction;Apoptosis; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids Organism Species: Mus musculus (Mouse) Detection Sensitivity: < 26.3pg/mL Detection Range: 62.50-4000pg/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEB343Mu.html