Mouse Apolipoprotein B (APOB) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Apolipoprotein B Alternative Names: Apo-B; FLDB; Apo B-100; Apo B-48 Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein B (APOB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein B (APOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein B (APOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein B (APOB) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: serum, plasma and other biological fluids Organism Species: Mus musculus (Mouse) Detection Sensitivity: < 2.63ng/mL Detection Range: 6.25-400ng/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEC003Mu.html