Human Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Secreted Frizzled Related Protein 1 Alternative Names: FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2 Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 1 (SFRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 1 (SFRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 1 (SFRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 1 (SFRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Signal transduction; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: serum, plasma, tissue homogenates and other biological fluids Organism Species: Homo sapiens (Human) Detection Sensitivity: < 0.057ng/mL Detection Range: 0.156-10ng/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEF880Hu.html