Supplier: Cloud-Clone Corp.
Product Type: ELISA Kit
Size: 96T
Item Name: A Disintegrin And Metalloproteinase With Thrombospondin 5
Alternative Names: ADMP-2; Aggrecanase-2
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Enzyme & Kinase;
Assay Type: Double-antibody Sandwich
Applications: Enzyme-linked immunosorbent assay for Antigen Detection.
Sample Types: tissue homogenates, cell lysates and other biological fluids
Organism Species: Homo sapiens (Human)
Detection Sensitivity: < 0.112ng/mL
Detection Range: 0.312-20ng/mL
Assay Length: 2 hours
Product Link: http://www.cloud-clone.com/products/SEK205Hu.html